Human CRP-Ab (C Reactive Protein Antibody) ELISA Kit-ExKits

INFORMATION

Full Name	C Reactive Protein Antibody
Synonym	CRP-Ab
Assay Type	Sandwich
Reactivity	Human
Range	7.81-500ng/mL
Sensitivity	4.69ng/mL
Sample Type	Serum, plasma and other biological fluids
Sample Volume	100μL
Detection Wavelength	OD450
Reaction Duration	3.25h
Transportation Temperature	2-8°C
Specificity	Specifically binds with CRP-Ab, no obvious cross reaction with other analogues.
Microplate	96-wells plate breakable into 12 x 8 wells strip

ELISA KIT COMPONENTS

Upon receipt, unpack promptly and store as recommended in the instructions.

Components	Specifications	Storage and Notes
Micro Plate	96T: 8 wells×12 strips 48T: 8 wells×6 strips	Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C.
Reference Standard	96T: 2 vials 48T: 1 vial	Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution.
Biotinylated Detection Ab Concentrate (100×)	96T: 120μL×1 vial 48T: 60μL×1 vial	Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution.
HRP Conjugate Concentrate (100×)	96T: 120μL×1 vial 48T: 60μL×1 vial	Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution.
Biotinylated Detection Ab Diluent	14mL×1	2-8℃, 12 months
HRP Conjugate Diluent	14mL×1	2-8℃, 12 months
Reference Standard & Sample Diluent	20mL×1	2-8℃, 12 months
Washing Buffer Concentrate (25×)	30mL×1	2-8℃, 12 months
Substrate Reagent(TMB)	10mL×1	2-8°C(Protect from light),12 months
Stop Solution	7mL×1	2-8°C/Room temperature

REQUIRED INSTRUMENTS AND REAGENTS

Microplate reader (450nm wavelength filter).
Incubator capable of maintaining 37℃.
1.5mL EP tube, absorbent paper.
Pipette and disposable tip: 0.5-10μL, 2-20μL, 20-200μL, 200-1000μL.
Double distilled water or deionized water.

ASSAY PROCEDURE SUMMARY

Add 100μL standard or sample to each well, incubate at 37℃ for 90 min. Discard the liquid.

Add 100μL Biotinlated Detection Ab working solution to each well. Incubate at 37℃ for 60 min.

Washing: Aspirate and wash the plate for 3 times.

Add 100μL HRP conjugate working solution. Incubate at 37℃ for 30 min.

Washing: Aspirate and wash the plate for 5 times.

Add 90μL Substrate Reagent. Incubate at 37℃ for 15 min.

Add 50μL Stop Solution.

Readings at 450nm. Calculation of results.

STANDARD CURVE

The product has been verified by the quality inspection department and meets the performance requirements of the product manual. (Laboratory temperature: 20-25℃, laboratory relative humidity: 20-60%. Before the experiment, all components were equilibrated to room temperature.) The following data is for reference only. A standard curve must be run with each test.

STD.(ng/mL)	OD-1	OD-2	Average
0	0.08	0.098	0.089
7.82	0.132	0.15	0.141
15.63	0.199	0.183	0.191
31.25	0.313	0.287	0.3
62.5	0.476	0.484	0.48
125	0.986	0.956	0.971
250	1.596	1.646	1.621
500	2.369	2.399	2.384

RECOVERY

The recovery of Human CRP-Ab spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

LINEARITY

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Human CRP-Ab in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.

PRECISION(%)

Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.