ExKits® PreGo™ Advantages
1 No Serial Dilution Required
Pre-loaded standard strip—no tedious serial dilution needed. Simply add diluent to generate the standard curve directly. Save time and effort with zero-barrier operation, making standard curve data acquisition effortless.
2 20% More Samples
Dedicated standard wells that don't occupy sample positions. Detect 20% more samples per plate, significantly boosting assay throughput.
Comprehensive Performance Highlights
【More | Full-Plate Throughput】
Dedicated independent standard curve strip design — no sample wells occupied by calibration standards. True 96-well full-plate assay capacity, delivering 20% higher sample throughput compared to conventional ELISA.
【Fast | Streamlined Workflow】
Eliminates tedious serial dilution protocols. No reconstitution or ratio-mixing required — simply add samples and start the assay, significantly reducing pre-experimental preparation time.
【Robust | Zero-Error Confidence】
Pre-formulated, dilution-free standard curve solution simplifies workflow with zero technical barrier. Minimizes experimental variability, extremely novice-friendly, ensuring effortless standard curve generation.
【Accurate | High-Quality & Reproducible】
Built upon classic, well-established ELISA reaction principles with strong anti-interference capability and excellent inter-assay reproducibility. Provides reliable data support for scientific research and grant project completion.
【Flexible | Dual-Mode Universal Compatibility】
Dual-mode design provides built-in flexibility: the standard pre-formulated zero-dilution standard curve enables rapid assay setup, while additional lyophilized reference standards are included as backup for manual serial dilution when needed. Seamlessly adapts to pilot experiments, batch testing, and multi-round validation workflows across all scenarios.
INFORMATION
| Full Name | @1@ |
|---|---|
| Synonym | @2@ |
| Assay Type | @3@ |
| Reactivity | @4@ |
| Range | @5@ |
| Sensitivity | @6@ |
| Sample Type | @7@ |
| Sample Volume | @8@ |
| Detection Wavelength | OD450 |
| Reaction Duration | @9@ |
| Transportation Temperature | 2-8°C |
| Specificity | Specifically binds with @10@, no obvious cross reaction with other analogues. |
| Microplate | Micro Plate: 96-wells plate breakable into 12 x 8 wells strip Standard Micro Plate: 2/3 x 8 wells strip |
ELISA KIT COMPONENTS
Upon receipt, unpack promptly and store as recommended in the instructions.
| Components | Specifications | Storage and Notes |
|---|---|---|
| Micro Plate | 96T: 8 wells×12 strips 48T: 8 wells×6 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Standard Micro Plate | 96T: 8 wells×3 strips 48T: 8 wells×2 strips | Unopened: -20°C, 12 months Unused: Put it back in the aluminum foil bag and seal it, store it at -20°C. |
| Reference Standard | 96T: 2 vials 48T: 1 vial | Unopened: -20°C, 12 months Please use freshly dissolved standards for each experiment. Discard any unused standards after dissolution. |
| Biotinylated Detection Ab Concentrate (100×) | 96T: 145μL×1 vial 48T: 75μL×1 vial | Unopened: -20°C, 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| HRP Conjugate Concentrate (100×) | 96T: 145μL×1 vial 48T: 75μL×1 vial | Unopened: -20°C(Protect from light), 12 months Unused: Please seal the concentrate and store it at -20°C, and discard the working solution. |
| Biotinylated Detection Ab Diluent | 15mL×1 | 2-8℃, 12 months |
| HRP Conjugate Diluent | 15mL×1 | 2-8℃, 12 months |
| Reference Standard & Sample Diluent | 20mL×1 | 2-8℃, 12 months |
| Washing Buffer Concentrate (25×) | 30mL×1 | 2-8℃, 12 months |
| Substrate Reagent(TMB) | 11mL×1 | 2-8°C(Protect from light),12 months |
| Stop Solution | 7mL×1 | 2-8°C/Room temperature |
REQUIRED INSTRUMENTS AND REAGENTS
- Microplate reader (450nm wavelength filter).
- Incubator capable of maintaining 37℃.
- 1.5mL EP tube, absorbent paper.
- Pipette and disposable tip: 0.5-10μL, 2-20μL, 20-200μL, 200-1000μL.
- Double distilled water or deionized water.
ASSAY PROCEDURE SUMMARY
@11@STANDARD CURVE
| STD.(@44@) | OD-1 | OD-2 | Average |
|---|---|---|---|
| 0 | @12@ | @13@ | @14@ |
| @15@ | @16@ | @17@ | @18@ |
| @19@ | @20@ | @21@ | @22@ |
| @23@ | @24@ | @25@ | @26@ |
| @27@ | @28@ | @29@ | @30@ |
| @31@ | @32@ | @33@ | @34@ |
| @35@ | @36@ | @37@ | @38@ |
| @39@ | @40@ | @41@ | @42@ |
RECOVERY
The recovery of @93@ spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
LINEARITY
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of @93@ in various matrices were diluted with the Reference Standard & Sample Diluent to produce samples with values within the dynamic range of the assay.
PRECISION(%)
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
| Item | Intra-assay Precision | Inter-assay Precision | ||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (@44@) | @45@ | @46@ | @47@ | @48@ | @49@ | @50@ |
| Standard deviation | @51@ | @52@ | @53@ | @54@ | @55@ | @56@ |
| CV(%) | @57@ | @58@ | @59@ | @60@ | @61@ | @62@ |
STABILITY
Perform stability testing of the kit at 37°C and 2-8°C and obtain relevant data.
| ELISA kit(n=5) | 37°C for 1 month | 2-8°C for 6 months |
|---|---|---|
| Average(%) | 80 | 95-100 |
